- Featured Product
- KD/KO Validated
TPI1 Polyclonal antibody
TPI1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells, rat liver tissue, rat kidney tissue, RAW 264.7 cells, mouse liver tissue, mouse kidney tissue, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TPI1 Ready-To-Use IHC Kit. TPI1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10713-1-AP targets TPI1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, yeast, pig, Drosophila |
| Immunogen | TPI1 fusion protein Ag1175 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | triosephosphate isomerase 1 |
| Synonyms | TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC007086 |
| Gene Symbol | TPI1 |
| Gene ID (NCBI) | 7167 |
| RRID | AB_2207716 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60174 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TPI1 antibody 10713-1-AP | Download protocol |
| IHC protocol for TPI1 antibody 10713-1-AP | Download protocol |
| IP protocol for TPI1 antibody 10713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Brain Amyloid-dependent triosephosphate isomerase nitrotyrosination induces glycation and tau fibrillation. |
