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  • Featured Product
  • KD/KO Validated

TPI1 Monoclonal antibody, PBS Only

TPI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7E5

Cat no : 67515-1-PBS

Print datasheet

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

验证数据展示

  • WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

    WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

    WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

    WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPI1 antibody included
  • IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

    IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPI1 Ready-To-Use IHC Kit. TPI1 primary antibody included.

产品信息

67515-1-PBS targets TPI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TPI1 fusion protein Ag16653 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name triosephosphate isomerase 1
Synonyms TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60174
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).