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  • Featured Product
  • KD/KO Validated

TPI1 Monoclonal antibody, PBS Only

TPI1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2E7E5

Cat No : 67515-1-PBS

Print datasheet

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

验证数据展示

  • WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

    WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

    WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

    WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPI1 antibody included
  • IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

    IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPI1 Ready-To-Use IHC Kit. TPI1 primary antibody included.

产品信息

67515-1-PBS targets TPI1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TPI1 fusion protein Ag16653 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name triosephosphate isomerase 1
Synonyms TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60174
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).