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TPM2 Polyclonal antibody

TPM2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55443-1-AP

Print datasheet

Synonyms

AMCD1, Beta tropomyosin, DA1, DA2B, TMSB, TPM2, Tropomyosin 2, tropomyosin 2 (beta), Tropomyosin beta chain

验证数据展示

  • WB analysis of mouse heart using 55443-1-AP

    WB analysis of mouse heart using 55443-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55443-1-AP (TPM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 55443-1-AP

    IHC staining of human breast cancer using 55443-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55443-1-AP (TPM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TPM2 antibody included
  • WB analysis of HeLa using 55443-1-AP

    WB analysis of HeLa using 55443-1-AP

    WB result of TPM2 antibody (55443-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM2 transfected HeLa cells.

经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TPM2 Ready-To-Use IHC Kit. TPM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55443-1-AP targets TPM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tropomyosin 2 (beta)
Synonyms AMCD1, Beta tropomyosin, DA1, DA2B, TMSB, TPM2, Tropomyosin 2, tropomyosin 2 (beta), Tropomyosin beta chain
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberNM_003289
Gene Symbol TPM2
Gene ID (NCBI) 7169
RRIDAB_2881335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07951
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPM2 antibody 55443-1-APDownload protocol
IHC protocol for TPM2 antibody 55443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols