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TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55444-1-AP

Print datasheet

Synonyms

Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain

验证数据展示

  • WB analysis of mouse skeletal muscle using 55444-1-AP

    WB analysis of mouse skeletal muscle using 55444-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55444-1-AP (TPM3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 55444-1-AP

    IHC staining of human colon using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 55444-1-AP

    IHC staining of human colon using 55444-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55444-1-AP (TPM3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, HepG2 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55444-1-AP targets TPM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tropomyosin 3
Synonyms Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberNM_153649
Gene Symbol TPM3
Gene ID (NCBI) 7170
RRIDAB_2881336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPM3 antibody 55444-1-APDownload protocol
IHC protocol for TPM3 antibody 55444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols