验证数据展示
产品信息
68251-1-PBS targets TPMT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, Indirect ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | TPMT fusion protein Ag28693 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | thiopurine S-methyltransferase |
| 别名 | Thiopurine methyltransferase, thiopurine S methyltransferase, TPMT |
| 计算分子量 | 28 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC005339 |
| 基因名称 | TPMT |
| Gene ID (NCBI) | 7172 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P51580 |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.