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  • Featured Product
  • KD/KO Validated

TPPII Monoclonal antibody, PBS Only

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H6F11

Cat no : 66017-1-PBS

Print datasheet

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

验证数据展示

  • WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

产品信息

66017-1-PBS targets TPPII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TPPII fusion protein Ag5296 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tripeptidyl peptidase II
Synonyms TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II
Calculated Molecular Weight 1249 aa, 138 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC039905
Gene Symbol TPPII
Gene ID (NCBI) 7174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).