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TPRKB Monoclonal antibody, PBS Only

TPRKB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5B9

Cat no : 66402-1-PBS

Print datasheet

Synonyms

TP53RK-binding protein, PRPK-binding protein, My019, EKC/KEOPS complex subunit TPRKB, CGI-121

验证数据展示

  • WB analysis of HeLa using 66402-1-Ig (same clone as 66402-1-PBS)

    WB analysis of HeLa using 66402-1-Ig (same clone as 66402-1-PBS)

    Western blot analysis of TPRKB in various cell lines using Proteintech antibody 66402-1-Ig at a dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

  • WB analysis using 66402-1-Ig (same clone as 66402-1-PBS)

    WB analysis using 66402-1-Ig (same clone as 66402-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66402-1-Ig (same clone as 66402-1-PBS)

    IHC staining of human skin cancer using 66402-1-Ig (same clone as 66402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66402-1-Ig (TPRKB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66402-1-Ig (same clone as 66402-1-PBS)

    IF Staining of Jurkat using 66402-1-Ig (same clone as 66402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TPRKB antibody (66402-1-Ig, Clone: 2D5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

产品信息

66402-1-PBS targets TPRKB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TPRKB fusion protein Ag18770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TP53RK binding protein
Synonyms TP53RK-binding protein, PRPK-binding protein, My019, EKC/KEOPS complex subunit TPRKB, CGI-121
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC029492
Gene Symbol TPRKB
Gene ID (NCBI) 51002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y3C4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues.