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验证数据展示

  • WB analysis of mouse lung using 55239-1-AP

    WB analysis of mouse lung using 55239-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 55239-1-AP (TPSD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 55239-1-AP

    IP experiment of A549 using 55239-1-AP

    IP result of anti-TPSD1 (IP:55239-1-AP, 3ug; Detection:55239-1-AP 1:300) with A549 cells lysate 920ug.

  • IHC staining of human lung cancer using 55239-1-AP

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55239-1-AP

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TPSD1 Polyclonal antibody

TPSD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 55239-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

Delta tryptase, HmMCP 3 like tryptase III, Mast cell mMCP 7 like, MCP7 LIKE, MCP7L1, MMCP 7L, TPSD1, Tryptase 3, Tryptase delta, tryptase delta 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55239-1-AP targets TPSD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tryptase delta 1
别名 Delta tryptase, HmMCP 3 like tryptase III, Mast cell mMCP 7 like, MCP7 LIKE, MCP7L1, MMCP 7L, TPSD1, Tryptase 3, Tryptase delta, tryptase delta 1
计算分子量 27 kDa
观测分子量 26-30 kDa
GenBank蛋白编号NM_012217
基因名称 TPSD1
Gene ID (NCBI) 23430
RRIDAB_2881293
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZJ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPSD1 antibody 55239-1-APDownload protocol
IHC protocol for TPSD1 antibody 55239-1-APDownload protocol
IP protocol for TPSD1 antibody 55239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols