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TPSD1 Polyclonal antibody

TPSD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55239-1-AP

Print datasheet

Synonyms

Delta tryptase, HmMCP 3 like tryptase III, Mast cell mMCP 7 like, MCP7 LIKE, MCP7L1, MMCP 7L, TPSD1, Tryptase 3, Tryptase delta, tryptase delta 1

验证数据展示

  • WB analysis of mouse lung using 55239-1-AP

    WB analysis of mouse lung using 55239-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 55239-1-AP (TPSD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 55239-1-AP

    IP experiment of A549 using 55239-1-AP

    IP result of anti-TPSD1 (IP:55239-1-AP, 3ug; Detection:55239-1-AP 1:300) with A549 cells lysate 920ug.

  • IHC staining of human lung cancer using 55239-1-AP

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 55239-1-AP

    IHC staining of human lung cancer using 55239-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55239-1-AP (TPSD1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55239-1-AP targets TPSD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tryptase delta 1
Synonyms Delta tryptase, HmMCP 3 like tryptase III, Mast cell mMCP 7 like, MCP7 LIKE, MCP7L1, MMCP 7L, TPSD1, Tryptase 3, Tryptase delta, tryptase delta 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberNM_012217
Gene Symbol TPSD1
Gene ID (NCBI) 23430
RRIDAB_2881293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPSD1 antibody 55239-1-APDownload protocol
IHC protocol for TPSD1 antibody 55239-1-APDownload protocol
IP protocol for TPSD1 antibody 55239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols