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TPST1 Polyclonal antibody

TPST1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12404-1-AP

Print datasheet

Synonyms

TPST 1, TPST1

验证数据展示

  • IHC staining of human colon cancer using 12404-1-AP

    IHC staining of human colon cancer using 12404-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12404-1-AP (TPST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 12404-1-AP

    WB analysis using 12404-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12404-1-AP (TPST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12404-1-AP targets TPST1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhamster
Immunogen TPST1 fusion protein Ag3093 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosylprotein sulfotransferase 1
Synonyms TPST 1, TPST1
Calculated Molecular Weight 370 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC013188
Gene Symbol TPST1
Gene ID (NCBI) 8460
RRIDAB_2877855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TPST1 antibody 12404-1-APDownload protocol
IHC protocol for TPST1 antibody 12404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
hamsterWB

Biotechnol J

Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation.

Authors - Ren Liu
View Article