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  • Featured Product
  • KD/KO Validated

TPX2 Polyclonal antibody, PBS Only

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11741-1-PBS

Print datasheet

Synonyms

DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2

验证数据展示

  • WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

    WB analysis using 11741-1-AP (same clone as 11741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human prostate cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    IHC staining of human colon cancer using 11741-1-AP (same clone as 11741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TPX2 antibody included
  • IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IP experiment of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:800) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    IF Staining of HeLa using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

    IF Staining of HepG2 using 11741-1-AP (same clone as 11741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 11741-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.

产品信息

11741-1-PBS targets TPX2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TPX2 fusion protein Ag2334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Synonyms DIL-2, DIL2, DIL 2, Differentially expressed in cancerous and non-cancerous lung cells 2, C20orf2
Calculated Molecular Weight 747 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).