验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, COLO 320 cells, HEK-293 cells, multi-cells, Raji cells, Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18099-1-AP targets TRAF3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, sheep |
| 免疫原 | TRAF3 fusion protein Ag12185 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TNF receptor-associated factor 3 |
| 别名 | CD40-binding protein, CD40 receptor-associated factor 1, CD40 binding protein, CAP-1, CAP1 |
| 计算分子量 | 568 aa, 64 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC075087 |
| 基因名称 | TRAF3 |
| Gene ID (NCBI) | 7187 |
| RRID | AB_10837364 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13114 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| IP protocol for TRAF3 antibody 18099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
| ||
Cell Death Differ The E3 ligase ASB3 downregulates antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation | ||
J Exp Med TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination. | ||
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response. | ||
EMBO J Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.
| ||
EMBO Rep Estrogen receptor alpha signaling in dendritic cells modulates autoimmune disease phenotype in mice |
