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  • Featured Product
  • KD/KO Validated

TRAF3 Monoclonal antibody, PBS Only

TRAF3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E3F4

Cat No : 66310-1-PBS

Print datasheet

Synonyms

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

验证数据展示

  • WB analysis using 66310-1-Ig (same clone as 66310-1-PBS)

    WB analysis using 66310-1-Ig (same clone as 66310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66310-1-Ig (TRAF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66310-1-Ig (same clone as 66310-1-PBS)

    WB analysis of HEK-293 using 66310-1-Ig (same clone as 66310-1-PBS)

    WB result of TRAF3 antibody (66310-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66310-1-Ig (TRAF3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    IHC staining of human lymphoma using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66310-1-Ig (TRAF3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66310-1-Ig (same clone as 66310-1-PBS)

    IF Staining of HeLa using 66310-1-Ig (same clone as 66310-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF3 antibody (66310-1-Ig, Clone: 1E3F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66310-1-PBS in a different storage buffer formulation.

产品信息

66310-1-PBS targets TRAF3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen TRAF3 fusion protein Ag12185 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 3
Synonyms CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3
Calculated Molecular Weight 568 aa, 64 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC075087
Gene Symbol TRAF3
Gene ID (NCBI) 7187
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13114
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5.