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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

    WB analysis using 66498-1-Ig (same clone as 66498-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66498-1-Ig (same clone as 66498-1-PBS)

    IF Staining of HeLa using 66498-1-Ig (same clone as 66498-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF6 antibody (66498-1-Ig, Clone: 1D1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66498-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRAF6 Monoclonal antibody, PBS Only

TRAF6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66498-1-PBS
CloneNo. 1D1E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNF85, RING-type E3 ubiquitin transferase TRAF6, Interleukin-1 signal transducer, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

产品信息

66498-1-PBS targets TRAF6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TRAF6 fusion protein Ag24696 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 6
Synonyms RNF85, RING-type E3 ubiquitin transferase TRAF6, Interleukin-1 signal transducer, EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6
Calculated Molecular Weight 522 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC031052
Gene Symbol TRAF6
Gene ID (NCBI) 7189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4K3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.