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  • Featured Product
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TRAFD1 Polyclonal antibody

TRAFD1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27741-1-AP

Print datasheet

Synonyms

TRAF-type zinc finger domain-containing protein 1, Protein FLN29, FLN29

验证数据展示

  • WB analysis of HEK-293 using 27741-1-AP

    WB analysis of HEK-293 using 27741-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27741-1-AP (TRAFD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 27741-1-AP

    WB analysis of A549 using 27741-1-AP

    WB result of TRAFD1 antibody (27741-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAFD1 transfected A549 cells.

  • IHC staining of human tonsillitis using 27741-1-AP

    IHC staining of human tonsillitis using 27741-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27741-1-AP

    IHC staining of human tonsillitis using 27741-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27741-1-AP (TRAFD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 27741-1-AP

    IP experiment of HEK-293 using 27741-1-AP

    IP result of anti-TRAFD1 (IP:27741-1-AP, 4ug; Detection:27741-1-AP 1:800) with HEK-293 cells lysate 1575 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27741-1-AP targets TRAFD1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen TRAFD1 fusion protein Ag26945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TRAF-type zinc finger domain containing 1
Synonyms TRAF-type zinc finger domain-containing protein 1, Protein FLN29, FLN29
Calculated Molecular Weight 582 aa, 65 kDa
Observed Molecular Weight 65-80 kDa
GenBank Accession NumberBC003553
Gene Symbol TRAFD1
Gene ID (NCBI) 10906
RRIDAB_2880958
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14545
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAFD1, also known as TRAF-type zinc finger domain containing 1, is a protein that plays a significant role in immune system signaling pathways. It was first identified as an interferon- (IFN) and lipopolysaccharide- (LPS) inducible factor. TRAFD1 contains a TRAF-type zinc finger domain at its N-terminus and a TRAF6-binding motif in its middle region, which allows it to interact with other TRAF proteins and modulate immune responses. In the context of immune signaling, TRAFD1 has been shown to suppress the inflammatory responses to innate immunity by inhibiting Toll-like receptor 4 (TLR4) dependent NF-κB and MAPK activation in monocytes/macrophages. This suggests that TRAFD1 can act as a negative regulator of immune signaling, dampening the inflammatory response. Moreover, TRAFD1 has been implicated in human diseases. It is overexpressed in many B cell-related cancers, and single nucleotide polymorphisms (SNPs) in TRAFD1 have been linked to non-Hodgkin's lymphoma.

实验方案

Product Specific Protocols
WB protocol for TRAFD1 antibody 27741-1-APDownload protocol
IHC protocol for TRAFD1 antibody 27741-1-APDownload protocol
IP protocol for TRAFD1 antibody 27741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols