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验证数据展示

  • WB analysis using 67693-1-Ig (same clone as 67693-1-PBS)

    WB analysis using 67693-1-Ig (same clone as 67693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    WB analysis of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 67693-1-Ig (same clone as 67693-1-PBS)

    IP experiment of K-562 using 67693-1-Ig (same clone as 67693-1-PBS)

    IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67693-1-Ig (same clone as 67693-1-PBS)

    IHC staining of mouse brain using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRAP1 Monoclonal antibody, PBS Only

TRAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 67693-1-PBS
CloneNo. 1H5D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67693-1-PBS targets TRAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRAP1 fusion protein Ag0400 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated protein 1
Synonyms TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75
Calculated Molecular Weight 80 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC000406
Gene Symbol TRAP1
Gene ID (NCBI) 10131
RRIDAB_2882886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12931
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).