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验证数据展示

  • WB analysis using 27774-1-AP

    WB analysis using 27774-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27774-1-AP (TRAPPC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 27774-1-AP

    IP experiment of HeLa using 27774-1-AP

    IP result of anti-TRAPPC11 (IP:27774-1-AP, 4ug; Detection:27774-1-AP 1:500) with HeLa cells lysate 1320 ug.

  • IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRAPPC11 Polyclonal antibody

TRAPPC11 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27774-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

C4orf41, UPF0636 protein C4orf41, Trafficking protein particle complex subunit 11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27774-1-AP targets TRAPPC11 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRAPPC11 fusion protein Ag27096 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 4 open reading frame 41
别名 C4orf41, UPF0636 protein C4orf41, Trafficking protein particle complex subunit 11
观测分子量129 kDa
GenBank蛋白编号BC022185
基因名称 TRAPPC11
Gene ID (NCBI) 60684
RRIDAB_3085995
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z392
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC11 is a subunit of the TRAPP (transport protein particle) tethering complex, which functions in intracellular vesicle trafficking. This subunit is involved in early stage endoplasmic reticulum-to-Golgi vesicle transport. Mutations in TRAPPC11 result in neuromuscular and developmental phenotypes.

实验方案

Product Specific Protocols
WB protocol for TRAPPC11 antibody 27774-1-APDownload protocol
IHC protocol for TRAPPC11 antibody 27774-1-APDownload protocol
IP protocol for TRAPPC11 antibody 27774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols