验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human liver tissue, human lung tissue, mouse brain tissue, mouse thymus tissue, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
12484-1-AP targets TRAPPC2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | TRAPPC2 fusion protein Ag3201 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | trafficking protein particle complex 2 |
| Synonyms | TRAPPC2B, TRAPPC2.19, Trafficking protein particle complex subunit 2B, SEDLP1, MIP-2A |
| Calculated Molecular Weight | 140 aa, 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC016915 |
| Gene Symbol | TRAPPC2 |
| Gene ID (NCBI) | 6399 |
| RRID | AB_2208136 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P0DI82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPPC2 antibody 12484-1-AP | Download protocol |
| IHC protocol for TRAPPC2 antibody 12484-1-AP | Download protocol |
| IP protocol for TRAPPC2 antibody 12484-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome. | ||
J Biol Chem The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis. | ||
iScience Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development |
