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TRAPPC4 Recombinant antibody

TRAPPC4 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

241158C11

Cat no : 84119-3-RR

Print datasheet

Synonyms

SBDN, PTD009, HSPC172, CGI-104, CGI 104

验证数据展示

  • WB analysis using 84119-3-RR

    WB analysis using 84119-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 84119-3-RR

    IP experiment of HL-60 using 84119-3-RR

    IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

  • Affinity and Kinetic Characterization of 84119-3-RR

    Affinity and Kinetic Characterization of 84119-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHCT 116 cells, HL-60 cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84119-3-RR targets TRAPPC4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TRAPPC4 fusion protein Ag2737 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name trafficking protein particle complex 4
Synonyms SBDN, PTD009, HSPC172, CGI-104, CGI 104
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC010866
Gene Symbol TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_3671682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y296
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

实验方案

Product Specific Protocols
WB protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
IP protocol for TRAPPC4 antibody 84119-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols