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TRAPPC9, NIBP Monoclonal antibody

TRAPPC9, NIBP Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C4F9

Cat No : 66131-1-Ig

Print datasheet

Synonyms

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRAPPC9,NIBP, TRS120, Tularik gene 1 protein

验证数据展示

  • WB analysis of human brain using 66131-1-Ig

    WB analysis of human brain using 66131-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66131-1-Ig (TRAPPC9,NIBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66131-1-Ig

    IHC staining of human kidney using 66131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66131-1-Ig (TRAPPC9, NIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66131-1-Ig is TRAPPC9, NIBP Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRAPPC9, NIBP fusion protein Ag8949 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name trafficking protein particle complex 9
Synonyms IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRAPPC9,NIBP, TRS120, Tularik gene 1 protein
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 128 kDa
GenBank Accession NumberBC006206
Gene Symbol TRAPPC9
Gene ID (NCBI) 83696
RRIDAB_2881530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96Q05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation and play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter.

实验方案

Product Specific Protocols
WB protocol for TRAPPC9, NIBP antibody 66131-1-IgDownload protocol
IHC protocol for TRAPPC9, NIBP antibody 66131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols