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TRAT1 Polyclonal antibody

TRAT1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13279-1-AP

Print datasheet

Synonyms

HSPC062, pp29/30, TCRIM, TRAT1

验证数据展示

  • WB analysis of mouse thymus using 13279-1-AP

    WB analysis of mouse thymus using 13279-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13279-1-AP (TRAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13279-1-AP

    IP experiment of Jurkat using 13279-1-AP

    IP result of anti-TRAT1 (IP:13279-1-AP, 4ug; Detection:13279-1-AP 1:300) with Jurkat cells lysate 3000 ug.

  • IHC staining of human tonsillitis using 13279-1-AP

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 13279-1-AP

    IHC staining of human tonsillitis using 13279-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13279-1-AP (TRAT1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13279-1-AP targets TRAT1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TRAT1 fusion protein Ag4023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T cell receptor associated transmembrane adaptor 1
Synonyms HSPC062, pp29/30, TCRIM, TRAT1
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC025713
Gene Symbol TRAT1
Gene ID (NCBI) 50852
RRIDAB_10643524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PIZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAT1 antibody 13279-1-APDownload protocol
IHC protocol for TRAT1 antibody 13279-1-APDownload protocol
IP protocol for TRAT1 antibody 13279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols