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验证数据展示

  • WB analysis using 66792-1-Ig (same clone as 66792-1-PBS)

    WB analysis using 66792-1-Ig (same clone as 66792-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66792-1-Ig (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TRBP Monoclonal antibody, PBS Only

TRBP Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66792-1-PBS

产品说明书

CloneNo. 1C9A10

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66792-1-PBS targets TRBP in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 TRBP fusion protein Ag8269 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TAR (HIV-1) RNA binding protein 2
别名 LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2
计算分子量 39 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC005860
基因名称 TRBP
Gene ID (NCBI) 6895
RRIDAB_2882136
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15633
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)