验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, SH-SY5Y cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26807-1-AP targets TREK1/KCNK2 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | TREK1/KCNK2 fusion protein Ag25209 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | potassium channel, subfamily K, member 2 |
别名 | hTREK 1c, hTREK 1e, K2p2.1, KCNK2, TPKC1, TREK, TREK 1, TREK 1 K(+) channel subunit, TREK1 |
计算分子量 | 411 aa, 46 kDa |
观测分子量 | 40-50 kDa |
GenBank蛋白编号 | BC101693 |
基因名称 | TREK1 |
Gene ID (NCBI) | 3776 |
RRID | AB_3085905 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95069 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The tandem of pore domains in a weak inward rectifying K+ channel (TWIK1, K2P1.1, or KCNK1) and TWIK-related K+channel 1 (TREK1, K2P 2.1, or KCNK2) are members of the two pore domain potassium (K2P) channel family, consisting of 15 channels that regulate the stabilization of resting membrane potential and cellular excitability by wielding background K+ leakage currents (PMID:12580339). TREK1/KCNK2 is sensitive to a wide range of physical and chemical cues. Its main role is to maintain the resting potential of the cell and whilst highly expressed in the nervous system, TREK1/KCNK2 is also expressed in the kidney, heart, lung and smooth muscle cells (PMID:31031627).
实验方案
Product Specific Protocols | |
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WB protocol for TREK1/KCNK2 antibody 26807-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |