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TREM2 Monoclonal antibody

TREM2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4H10

Cat No : 68723-1-Ig

Print datasheet

Synonyms

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

验证数据展示

  • WB analysis using 68723-1-Ig

    WB analysis using 68723-1-Ig

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

  • IHC staining of mouse brain using 68723-1-Ig

    IHC staining of mouse brain using 68723-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TREM2 antibody included
  • IHC staining of mouse brain using 68723-1-Ig

    IHC staining of mouse brain using 68723-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TREM2 antibody included

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TREM2 Ready-To-Use IHC Kit. TREM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68723-1-Ig targets TREM2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen TREM2 fusion protein Ag6843 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name triggering receptor expressed on myeloid cells 2
Synonyms TREM 2, TREM2, Trem2a, Trem2b, Trem2c
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight26-32 kDa
GenBank Accession NumberBC032362
Gene Symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).

实验方案

Product Specific Protocols
WB protocol for TREM2 antibody 68723-1-IgDownload protocol
IHC protocol for TREM2 antibody 68723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols