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TREM2 Recombinant antibody

TREM2 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240384B7

Cat no : 83438-6-RR

Print datasheet

Synonyms

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

验证数据展示

  • WB analysis of THP-1 using 83438-6-RR

    WB analysis of THP-1 using 83438-6-RR

    PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 83438-6-RR

    WB analysis of THP-1 using 83438-6-RR

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83438-6-RR

    Affinity and Kinetic Characterization of 83438-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83438-6-RR against Human TREM2 were performed. The affinity constant is 38.7 nM.

经过测试的应用

Positive WB detected inPMA treated THP-1 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy TREM2 Ready-To-Use IHC Kit. TREM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83438-6-RR is TREM2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name triggering receptor expressed on myeloid cells 2
Synonyms Trem2c, Trem2b, Trem2a, TREM-2, TREM 2
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight26-32 kDa
GenBank Accession NumberBC032362
Gene Symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NZC2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

实验方案

Product Specific Protocols
WB protocol for TREM2 antibody 83438-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols