验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
20062-1-AP targets TRERF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcriptional regulating factor 1 |
| 别名 | HSA277276, RAPA, RP1 139D8.5, TReP 132, TREP132, TRERF1, Zinc finger protein rapa |
| 计算分子量 | 132 kDa |
| 观测分子量 | 132-140 kDa |
| GenBank蛋白编号 | NM_033502 |
| 基因名称 | TRERF1 |
| Gene ID (NCBI) | 55809 |
| RRID | AB_10666203 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PN7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRERF1, also named as BCAR2, RAPA and TREP132, activates transcription of CYP11A1. TRERF1 interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. The antibody recognizes the C-term of TRERF1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRERF1 antibody 20062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
