TRH Polyclonal antibody
TRH Polyclonal Antibody for WB, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF-P, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, BxPC-3 cells |
Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25332-1-AP targets TRH in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | TRH fusion protein Ag12623 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | thyrotropin-releasing hormone |
Synonyms | Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor |
Calculated Molecular Weight | 242 aa, 27 kDa |
Observed Molecular Weight | 27-33 kDa |
GenBank Accession Number | BC074889 |
Gene Symbol | TRH |
Gene ID (NCBI) | 7200 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20396 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).
实验方案
Product Specific Protocols | |
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WB protocol for TRH antibody 25332-1-AP | Download protocol |
IF protocol for TRH antibody 25332-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |