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TRH Polyclonal antibody

TRH Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25332-1-AP

Print datasheet

Synonyms

Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor

验证数据展示

  • WB analysis using 25332-1-AP

    WB analysis using 25332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25332-1-AP (TRH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 25332-1-AP

    IF Staining of mouse cerebellum using 25332-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using TRH antibody (25332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, BxPC-3 cells
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25332-1-AP targets TRH in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRH fusion protein Ag12623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyrotropin-releasing hormone
Synonyms Prothyroliberin, Protirelin, Thyrotropin releasing factor, thyrotropin releasing hormone, TRF, TRH, TSH releasing factor
Calculated Molecular Weight 242 aa, 27 kDa
Observed Molecular Weight27-33 kDa
GenBank Accession NumberBC074889
Gene Symbol TRH
Gene ID (NCBI) 7200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).

实验方案

Product Specific Protocols
WB protocol for TRH antibody 25332-1-APDownload protocol
IF protocol for TRH antibody 25332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols