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TRIB2 Polyclonal antibody

TRIB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15359-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, mouse thymus tissue, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15359-1-AP targets TRIB2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TRIB2 fusion protein Ag7605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tribbles homolog 2 (Drosophila)
别名 C5FW, GS3955, TRB 2, TRB2, TRIB2, Tribbles homolog 2
计算分子量 39 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002637
基因名称 TRIB2
Gene ID (NCBI) 28951
RRIDAB_2209083
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIB2, also named as TRB2 , belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and Tribbles subfamily. TRIB2 interacts with MAPK kinases and regulates activation of MAP kinases. It does not display kinase activity. Antibodies against TRIB2 are present in sera from patients with autoimmune uveitis(uniprot). TRIB2 is enriched in hypocretin neurons. Sera from narcolepsy patients with cataplexy had higher Trib2-specific antibody titers compared with either normal controls or patients with idiopathic hypersomnia, multiple sclerosis, or other inflammatory neurological disorders.

实验方案

Product Specific Protocols
WB protocol for TRIB2 antibody 15359-1-APDownload protocol
IHC protocol for TRIB2 antibody 15359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

iScience

FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons

Authors - Wan Yun Ho
mouseWB,IF

Exp Mol Med

Trib2 regulates the pluripotency of embryonic stem cells and enhances reprogramming efficiency.

Authors - Eun Kyoung Do
  • KD Validated
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