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验证数据展示

  • WB analysis using 84651-3-RR

    WB analysis using 84651-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84651-3-RR (TRIM11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84651-3-RR

    Affinity and Kinetic Characterization of 84651-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84651-3-RR against Human TRIM11 were performed. The affinity constant is 0.25 nM.

TRIM11 Recombinant antibody

TRIM11 Recombinant Antibody for WB, ELISA
Cat No. 84651-3-RR

产品说明书

CloneNo. 242107F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RING finger protein 92, Protein BIA1, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM11, BIA1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84651-3-RR targets TRIM11 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 TRIM11 fusion protein Ag1308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 11
别名 RING finger protein 92, Protein BIA1, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM11, BIA1
计算分子量 52 kDa
观测分子量 53 kDa
GenBank蛋白编号BC011629
基因名称 TRIM11
Gene ID (NCBI) 81559
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96F44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM11(Tripartite motif-containing protein 11) is also named as RNF92 and Belongs to the TRIM/RBCC family. It is a member of the tripartite-motif-containing protein family and is known to destabilize humanin, an inhibitor of Alzheimer-like neuronal insults(PMID: 16904669). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TRIM11 antibody 84651-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols