• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

TRIM25 Polyclonal antibody

TRIM25 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12573-1-AP

Print datasheet

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

验证数据展示

  • WB analysis using 12573-1-AP

    WB analysis using 12573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 12573-1-AP

    IHC staining of human kidney using 12573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM25 antibody included
  • IHC staining of human kidney using 12573-1-AP

    IHC staining of human kidney using 12573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM25 antibody included
  • IP experiment of K-562 using 12573-1-AP

    IP experiment of K-562 using 12573-1-AP

    IP result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.

  • IF Staining of HeLa using 12573-1-AP

    IF Staining of HeLa using 12573-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 12573-1-AP

    IF Staining of HeLa using 12573-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue, mouse pancreas tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12573-1-AP targets TRIM25 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TRIM25 fusion protein Ag3241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 25
Synonyms EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68-71 kDa
GenBank Accession NumberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
RRIDAB_2209732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

实验方案

Product Specific Protocols
WB protocol for TRIM25 antibody 12573-1-APDownload protocol
IHC protocol for TRIM25 antibody 12573-1-APDownload protocol
IF protocol for TRIM25 antibody 12573-1-APDownload protocol
IP protocol for TRIM25 antibody 12573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Signal Transduct Target Ther

Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis

Authors - Yuanliang Yan
View Article
human,mouseWB

Nat Immunol

USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation

Authors -
  • KO Validated
View Article
mouseWB

Nat Immunol

Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.

Authors - Wang Penghua P
View Article
humanWB

Cell Rep

p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP

Authors - Yingdan Huang
View Article
humanWB

Elife

Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.

Authors - Wei Liu
View Article
humanWB

Haematologica

Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.

Authors - Jia Tong
View Article