• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
  • Featured Product
  • KD/KO验证

TRIM25 Monoclonal antibody, PBS Only

TRIM25 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67314-1-PBS

产品说明书

CloneNo. 1H6G1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.

产品信息

67314-1-PBS targets TRIM25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 TRIM25 fusion protein Ag29325 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 25
别名 EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
计算分子量 630 aa, 71 kDa
观测分子量 68-71 kDa
GenBank蛋白编号BC016924
基因名称 TRIM25
Gene ID (NCBI) 7706
RRIDAB_2882574
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14258
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).