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  • Featured Product
  • KD/KO Validated

TRIM25 Monoclonal antibody, PBS Only

TRIM25 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H6G1

Cat no : 67314-1-PBS

Print datasheet

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

验证数据展示

  • IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    IF Staining of HEK-293T using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    WB analysis using 67314-1-Ig (same clone as 67314-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
  • IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    IHC staining of human breast cancer using 67314-1-Ig (same clone as 67314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67314-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TRIM25 antibody included
Planning an IHC experiment? We recommend our IHCeasy TRIM25 Ready-To-Use IHC Kit. TRIM25 primary antibody included.

产品信息

67314-1-PBS targets TRIM25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TRIM25 fusion protein Ag29325 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 25
Synonyms EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 68-71 kDa
GenBank Accession NumberBC016924
Gene Symbol TRIM25
Gene ID (NCBI) 7706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14258
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).