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  • Featured Product
  • KD/KO Validated

KAP1 Polyclonal antibody

KAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 15202-1-AP

Print datasheet

Synonyms

TRIM28, KRAB interacting protein 1, KRAB associated protein 1, KAP-1, KAP 1

验证数据展示

  • WB analysis of HeLa using 15202-1-AP

    WB analysis of HeLa using 15202-1-AP

    WB result of KAP1 antibody (15202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HeLa cells.

  • IP experiment of HeLa using 15202-1-AP

    IP experiment of HeLa using 15202-1-AP

    IP result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • WB analysis using 15202-1-AP

    WB analysis using 15202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15202-1-AP (KAP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 15202-1-AP

    IHC staining of human colon using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IHC staining of human lung cancer using 15202-1-AP

    IHC staining of human lung cancer using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IHC staining of human lung cancer using 15202-1-AP

    IHC staining of human lung cancer using 15202-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15202-1-AP (KAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAP1 antibody included
  • IF Staining of HeLa using 15202-1-AP

    IF Staining of HeLa using 15202-1-AP

    Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 15202-1-AP

    IF Staining of HeLa using 15202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KAP1 antibody (15202-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 15202-1-AP

    FC experiment of HeLa using 15202-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KAP1 (15202-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KAP1 Ready-To-Use IHC Kit. KAP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15202-1-AP targets KAP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, chIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, monkey
Immunogen KAP1 fusion protein Ag7350 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 28
Synonyms TRIM28, KRAB interacting protein 1, KRAB associated protein 1, KAP-1, KAP 1
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC004978
Gene Symbol KAP1
Gene ID (NCBI) 10155
RRIDAB_2209890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

实验方案

Product Specific Protocols
WB protocol for KAP1 antibody 15202-1-APDownload protocol
IHC protocol for KAP1 antibody 15202-1-APDownload protocol
IF protocol for KAP1 antibody 15202-1-APDownload protocol
IP protocol for KAP1 antibody 15202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding
View Article
mouseWB, IHC

Hepatology

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Authors - Bojkowska Karolina K
  • KO Validated
View Article
mouseWB,IP

J Exp Med

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Authors - Longfei Ma
View Article
mouseWB

Nucleic Acids Res

Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.

Authors - Weihua Qin
  • KO Validated
View Article
humanchIP

Proc Natl Acad Sci U S A

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

Authors - Min Li
View Article
mouseWB,ChIP

Cell Rep

POGZ suppresses 2C transcriptional program and retrotransposable elements

Authors - Xiaoyun Sun
View Article