验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, BxPC-3 cells |
| Positive IHC detected in | human skin cancer tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17542-1-AP targets TRIM29 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | TRIM29 fusion protein Ag11600 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 29 |
| 别名 | ATDC, TRIM29, tripartite motif containing 29 |
| 计算分子量 | 588 aa, 66 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC017352 |
| 基因名称 | TRIM29 |
| Gene ID (NCBI) | 23650 |
| RRID | AB_2272412 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14134 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein).ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1(PMID:7644499). ATDC gene is highly expressed in samples of primary pancreatic cancer and PanIN lesions compared to normal pancreas and chronic pancreatitis and the highly expressed protein in human pancreatic cancer,enhances cancer cell proliferation and in vivo tumorigenicity by upregulation of the Wnt/b-Catenin signaling pathway(Wang, L. etc.2007).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM29 antibody 17542-1-AP | Download protocol |
| IHC protocol for TRIM29 antibody 17542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.
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Oncogenesis Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation. | ||
PLoS One Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway. | ||
Cancer Sci Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. | ||
Cancer Biol Ther iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma. | ||
Cancer Res Treat TRIM29 Overexpression Promotes Proliferation and Survival of Bladder Cancer Cells Through NF-κB Signaling.
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