验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
| Positive IP detected in | HT-29 cells |
| Positive IHC detected in | human small intestine tissue, Human Colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12543-1-AP targets TRIM31 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | TRIM31 fusion protein Ag3264 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 31 |
| 别名 | TRIM, RNF, HCGI, HCG1, EC:2.3.2.27 |
| 计算分子量 | 425 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC017017 |
| 基因名称 | TRIM31 |
| Gene ID (NCBI) | 11074 |
| RRID | AB_10640897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZY9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM31(Tripartite motif-containing protein 31) is also named as C6orf13, RNF and belongs to the TRIM/RBCC family. The deduced protein contains an N-terminal RING domain, a B-box-2 domain, and a coiled-coil region. TRIM31 forms homodimers(PMID:11331580). This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| IHC protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| IP protocol for TRIM31 antibody 12543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity.
| ||
Nat Commun USP18 positively regulates innate antiviral immunity by promoting K63-linked polyubiquitination of MAVS. | ||
Redox Biol The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.
| ||
Cells Inhibition of Immunoproteasome Attenuates NLRP3 Inflammasome Response by Regulating E3 Ubiquitin Ligase TRIM31 | ||
J Virol TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. |