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验证数据展示

  • WB analysis of K-562 using 83316-2-RR

    WB analysis of K-562 using 83316-2-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 83316-2-RR

    WB analysis of human placenta using 83316-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 83316-2-RR

    IF Staining of HEK-293 using 83316-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83316-2-RR

    Affinity and Kinetic Characterization of 83316-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

TRIM34 Recombinant antibody

TRIM34 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83316-2-RR

产品说明书

CloneNo. 240180E2

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, human placenta tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83316-2-RR targets TRIM34 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 TRIM34 fusion protein Ag34444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tripartite motif-containing 34
别名 IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34
计算分子量57 kDa
观测分子量31 kDa, 57 kDa
GenBank蛋白编号NM_001003827
基因名称 TRIM34
Gene ID (NCBI) 53840
RRIDAB_3670982
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYJ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).

实验方案

Product Specific Protocols
WB protocol for TRIM34 antibody 83316-2-RRDownload protocol
IF protocol for TRIM34 antibody 83316-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols