TRIM40 Monoclonal antibody
TRIM40 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C5D3
验证数据展示
经过测试的应用
| Positive WB detected in | NCCIT cell, THP-1 cells, pig small intestine tissue, HT-29 cells, NCCIT cells, JAR cells |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67073-1-Ig targets TRIM40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Immunogen | TRIM40 fusion protein Ag21660 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | tripartite motif-containing 40 |
| Synonyms | TRIM, RNF35, Probable E3 NEDD8-protein ligase, EC:2.3.2.27, E3 ubiquitin ligase TRIM40 |
| Calculated Molecular Weight | 258 aa, 29 kDa |
| Observed Molecular Weight | 26-36 kDa |
| GenBank Accession Number | BC060785 |
| Gene Symbol | TRIM40 |
| Gene ID (NCBI) | 135644 |
| RRID | AB_2882382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6P9F5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM40 antibody 67073-1-Ig | Download protocol |
| IHC protocol for TRIM40 antibody 67073-1-Ig | Download protocol |
| IF protocol for TRIM40 antibody 67073-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner | ||
Nat Commun EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses |
