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验证数据展示

  • WB analysis using 31053-1-AP

    WB analysis using 31053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31053-1-AP (TRIM43 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 31053-1-AP

    FC experiment of A549 using 31053-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.4 ug TRIM43 Polyclonal antibody (31053-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TRIM43 Polyclonal antibody

TRIM43 Polyclonal Antibody for WB, ELISA
Cat No. 31053-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Tripartite motif-containing protein 43, TRIM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, Jurkat cells, U-87 MG cells, mouse brain tissue
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31053-1-AP targets TRIM43 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRIM43 fusion protein Ag34926 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 43
Synonyms Tripartite motif-containing protein 43, TRIM
Observed Molecular Weight52-60 kDa
GenBank Accession NumberBC015353
Gene Symbol TRIM43
Gene ID (NCBI) 129868
RRIDAB_3669834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BQ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM43 (tripartite motif containing 43), also known as TRIM43A. It is expected to be located in the cytoplasm. The calculated molecular weight of TRIM43 is 52 kDa. TRIM43 was also upregulated in herpesvirus-associated diseases in humans, including γ herpesvirus-associated tumors. As such, TRIM43 and its transcription factor DUX4 could potentially serve as biomarkers of active herpesviral replication and pathogenesis (PMID: 30420784).

实验方案

Product Specific Protocols
WB protocol for TRIM43 antibody 31053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols