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TRIM46 Polyclonal antibody

TRIM46 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30298-1-AP

Print datasheet

Synonyms

FLJ23229, Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

验证数据展示

  • WB analysis using 30298-1-AP

    WB analysis using 30298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30298-1-AP (TRIM46 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30298-1-AP

    IF Staining of HepG2 using 30298-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (30298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30298-1-AP targets TRIM46 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse, rat, human
Cited Reactivitymouse
Immunogen TRIM46 fusion protein Ag33175 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 46
Synonyms FLJ23229, Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46
Calculated Molecular Weight 759 aa, 83 kDa
Observed Molecular Weight83 kDa
GenBank Accession NumberBC069416
Gene Symbol TRIM46
Gene ID (NCBI) 80128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

实验方案

Product Specific Protocols
WB protocol for TRIM46 antibody 30298-1-APDownload protocol
IF protocol for TRIM46 antibody 30298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination

Authors - Anja Konietzny
View Article