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TRIM46 Recombinant antibody

TRIM46 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240557E1

Cat no : 83614-2-RR

Print datasheet

Synonyms

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

验证数据展示

  • WB analysis using 83614-2-RR

    WB analysis using 83614-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 83614-2-RR

    WB analysis of U-87 MG using 83614-2-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83614-2-RR

    IF Staining of A549 using 83614-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HeLa using 83614-2-RR

    FC experiment of HeLa using 83614-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83614-2-RR

    Affinity and Kinetic Characterization of 83614-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inSH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83614-2-RR is TRIM46 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TRIM46 fusion protein Ag15290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tripartite motif-containing 46
Synonyms Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY
Calculated Molecular Weight 759 aa, 83 kDa
Observed Molecular Weight83 kDa
GenBank Accession NumberBC069416
Gene Symbol TRIM46
Gene ID (NCBI) 80128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z4K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

实验方案

Product Specific Protocols
WB protocol for TRIM46 antibody 83614-2-RRDownload protocol
IF protocol for TRIM46 antibody 83614-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols