验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, U-87 MG cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83614-2-RR targets TRIM46 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | TRIM46 fusion protein Ag15290 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | tripartite motif-containing 46 |
| Synonyms | Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY |
| Calculated Molecular Weight | 759 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC069416 |
| Gene Symbol | TRIM46 |
| Gene ID (NCBI) | 80128 |
| RRID | AB_3671227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z4K8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM46 antibody 83614-2-RR | Download protocol |
| IHC protocol for TRIM46 antibody 83614-2-RR | Download protocol |
| IF protocol for TRIM46 antibody 83614-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |