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  • KD/KO Validated

TRIM47 Polyclonal antibody

TRIM47 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26885-1-AP

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Synonyms

GOA, RING finger protein 100, RNF100, TRIM47, tripartite motif containing 47



经过测试的应用

Positive WB detected inU2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman kidney tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRIM47 Ready-To-Use IHC Kit. TRIM47 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26885-1-AP is TRIM47 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen TRIM47 fusion protein Ag22868 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 47
Synonyms GOA, RING finger protein 100, RNF100, TRIM47, tripartite motif containing 47
Calculated Molecular Weight 638 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC017299
Gene Symbol TRIM47
Gene ID (NCBI) 91107
RRIDAB_2880669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LD4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for TRIM47 antibody 26885-1-APDownload protocol
IHC protocol for TRIM47 antibody 26885-1-APDownload protocol
IP protocol for TRIM47 antibody 26885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Neoplasma

Trim47 overexpression correlates with poor prognosis in gastric cancer.

Authors - YuJian Xia
human,mouseWB,IHC,IP

Signal Transduct Target Ther

TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.

Authors - Yisong Qian
  • KD Validated
humanWB,IHC

In Vitro Cell Dev Biol Anim

TRIM47 promotes glioma angiogenesis by suppressing Smad4

Authors - Zifan Wang
  • KD Validated
human,mouseWB,IHC,IF

Clinics (Sao Paulo)

TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling

Authors - Xi Wang
  • KD Validated
ratWB

Exp Brain Res

Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats

Authors - Yingjun Zhu
  • KD Validated
humanWB,IF

Comput Struct Biotechnol J

Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress

Authors - Rui Su