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验证数据展示

  • WB analysis using 27274-1-AP

    WB analysis using 27274-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27274-1-AP (TRIM55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27274-1-AP

    IHC staining of human liver cancer using 27274-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27274-1-AP (TRIM55 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27274-1-AP

    IF Staining of HeLa using 27274-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM55 antibody (27274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

TRIM55 Polyclonal antibody

TRIM55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27274-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MURF 2, MuRF2, RING finger protein 29, RNF29, TRIM55, tripartite motif containing 55

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inC2C12 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27274-1-AP targets TRIM55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TRIM55 fusion protein Ag25338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 55
Synonyms MURF 2, MuRF2, RING finger protein 29, RNF29, TRIM55, tripartite motif containing 55
Calculated Molecular Weight 548 aa, 60 kDa
Observed Molecular Weight60-65 kDa，75-80 kDa
GenBank Accession NumberBC007750
Gene Symbol TRIM55
Gene ID (NCBI) 84675
RRIDAB_3669590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing 55 (TRIM55), which is also called muscle-specific ring finger 2 (Murf2), has been indicated to participate in the natural development of muscle and the cardiovascular system. The expression of TRIM55 also inhibited several markers of cancer stem-like cells (PMID: 34974792). MURF2 mRNA studies revealed up to four potential isoforms generated by differential splicing events within the 3′-region of the gene: p60, p50, p27, and p60B MURF2 isoforms. The change in the ratio of E3 ligase isoforms regulates the sequential activation of proteasomal or autophagic degradation of target components(PMID: 15199100, PMID: 24124537). This antibody could detect multiple isoforms or post-translational modifications of TRIM55.

实验方案

Product Specific Protocols
WB protocol for TRIM55 antibody 27274-1-APDownload protocol
IHC protocol for TRIM55 antibody 27274-1-APDownload protocol
IF protocol for TRIM55 antibody 27274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols