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验证数据展示

  • WB analysis using 28575-1-AP

    WB analysis using 28575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28575-1-AP (TRIM59 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 28575-1-AP

    IHC staining of human pancreas cancer using 28575-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM59 antibody included
  • IF Staining of U2OS using 28575-1-AP

    IF Staining of U2OS using 28575-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM59 antibody (28575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 28575-1-AP

    IHC staining of human lung cancer using 28575-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM59 antibody included
  • IHC staining of human liver using 28575-1-AP

    IHC staining of human liver using 28575-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRIM59 antibody included
  • IHC staining of human breast cancer using 28575-1-AP

    IHC staining of human breast cancer using 28575-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28575-1-AP (TRIM59 antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with TRIM59 antibody included
  • Featured Product
  • KD/KO Validated

TRIM59 Polyclonal antibody

TRIM59 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28575-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Synonyms

MRF1, RING finger protein 104, RNF104, TRIM57, TRIM59, tripartite motif containing 59, TSBF1, Tumor suppressor TSBF 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inhuman pancreas cancer tissue, human liver tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TRIM59 Ready-To-Use IHC Kit. TRIM59 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28575-1-AP targets TRIM59 in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, CoIP, ChIP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen TRIM59 fusion protein Ag29560 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 59
Synonyms MRF1, RING finger protein 104, RNF104, TRIM57, TRIM59, tripartite motif containing 59, TSBF1, Tumor suppressor TSBF 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberNM_173084
Gene Symbol TRIM59
Gene ID (NCBI) 286827
RRIDAB_2918177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM59 belongs to the tripartite motif (TRIM) family. It functions as E3 ubiquitin ligases or an adaptor protein, and plays important roles in various types of human cancers.

实验方案

Product Specific Protocols
WB protocol for TRIM59 antibody 28575-1-APDownload protocol
IHC protocol for TRIM59 antibody 28575-1-APDownload protocol
IF protocol for TRIM59 antibody 28575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP,ChIP

J Transl Med

A role of TRIM59 in pulmonary hypertension: modulating the protein ubiquitylation modification

Authors - Yingli Liu
View Article
humanWB,CoIP

Heliyon

TRIM59-mediated ferroptosis enhances neuroblastoma development and chemosensitivity through p53 ubiquitination and degradation

Authors - Yingbei Liu
  • KD Validated
View Article