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  • KD/KO Validated

TRIM72 Polyclonal antibody

TRIM72 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22151-1-AP

Print datasheet

Synonyms

hMG53, Mg53, Mitsugumin 53, TRIM72, tripartite motif containing 72



经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22151-1-AP targets TRIM72 in WB, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TRIM72 fusion protein Ag17756 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 72
Synonyms hMG53, Mg53, Mitsugumin 53, TRIM72, tripartite motif containing 72
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033211
Gene Symbol TRIM72
Gene ID (NCBI) 493829
RRIDAB_2879009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZMU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRIM72 antibody 22151-1-APDownload protocol
IF protocol for TRIM72 antibody 22151-1-APDownload protocol
IP protocol for TRIM72 antibody 22151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Int J Mol Sci

PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice

Authors - Baile Wu
mouseWB

Int Immunopharmacol

MG53 binding to CAV3 facilitates activation of eNOS/NO signaling pathway to enhance the therapeutic benefits of bone marrow-derived mesenchymal stem cells in diabetic wound healing

Authors - Junwei Wu
humanWB,IP

Cancer Biomark

Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment

Authors - Xun Li
  • KD Validated
ratWB

Biochem Biophys Res Commun

Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway.

Authors - Jiang Li
ratWB

BMC Cardiovasc Disord

Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner.

Authors - Jiawei Zhuang
humanWB

FASEB J

Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts

Authors - Yan Li
  • KD Validated