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TRIM9 Monoclonal antibody, PBS Only

TRIM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2B10

Cat no : 67268-1-PBS

Print datasheet

Synonyms

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

验证数据展示

  • WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

    WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

产品信息

67268-1-PBS targets TRIM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen TRIM9 fusion protein Ag27856 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 9
Synonyms KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 90 kDa, 79 kDa, 61 kDa
GenBank Accession NumberBC013414
Gene Symbol TRIM9
Gene ID (NCBI) 114088
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9C026
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.