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TRIP6 Monoclonal antibody

TRIP6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D8C7

Cat no : 60205-1-Ig

Print datasheet

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

验证数据展示

  • WB analysis of HeLa using 60205-1-Ig

    WB analysis of HeLa using 60205-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60205-1-Ig (TRIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 60205-1-Ig

    IHC staining of human ovary tumor using 60205-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60205-1-Ig (TRIP6 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHela cells
Positive IHC detected inhuman ovary tumor tissue, human liver tissue, human colon cancer tissue, human colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60205-1-Ig targets TRIP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Immunogen TRIP6 fusion protein Ag15149 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name thyroid hormone receptor interactor 6
Synonyms OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1
Calculated Molecular Weight 476 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC004249
Gene Symbol TRIP6
Gene ID (NCBI) 7205
RRIDAB_10973674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15654
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

实验方案

Product Specific Protocols
WB protocol for TRIP6 antibody 60205-1-IgDownload protocol
IHC protocol for TRIP6 antibody 60205-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Dev Cell

Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex.

Authors - Marcela Raices
View Article