验证数据展示
经过测试的应用
| Positive WB detected in | mouse placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17141-1-AP targets TRIT1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | TRIT1 fusion protein Ag10906 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tRNA isopentenyltransferase 1 |
| 别名 | hGRO1, IPP transferase, IPPT, IPT, IPTase, MOD5, TRIT1, tRNA isopentenyltransferase, tRNA isopentenyltransferase 1 |
| 计算分子量 | 467 aa, 53 kDa |
| 观测分子量 | 53-60 kDa |
| GenBank蛋白编号 | BC107569 |
| 基因名称 | TRIT1 |
| Gene ID (NCBI) | 54802 |
| RRID | AB_3669261 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3H1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IPTases (TRIT1) belong to a group of enzymes that modify both cytoplasmic and mitochondrial tRNAs of eukaryotic cells. Pathogenic mutations to human IPTase (TRIT1) cause mitochondrial insufficiency and neurodevelopmental disease, principally due to decreased modification of the mt-tRNA substrates.(PMID: 32324744, PMID: 36049610)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIT1 antibody 17141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |