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验证数据展示

  • WB analysis of HeLa using 14970-1-AP

    WB analysis of HeLa using 14970-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14970-1-AP (TRMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14970-1-AP

    IHC staining of human colon cancer using 14970-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 14970-1-AP

    IHC staining of human colon cancer using 14970-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 14970-1-AP

    IF Staining of HeLa using 14970-1-AP

    Immunofluorescent analysis of HeLa cells using 14970-1-AP (TRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TRMT1 Polyclonal antibody

TRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14970-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

tRNA(guanine-26,N(2)-N(2)) methyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA (guanine(26)-N(2))-dimethyltransferase, TRMT 1, TRM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14970-1-AP targets TRMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRMT1 fusion protein Ag6837 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
别名 tRNA(guanine-26,N(2)-N(2)) methyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA (guanine(26)-N(2))-dimethyltransferase, TRMT 1, TRM1
计算分子量 81 kDa
观测分子量 72 kDa
GenBank蛋白编号BC002492
基因名称 TRMT1
Gene ID (NCBI) 55621
RRIDAB_2209504
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXH9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

实验方案

Product Specific Protocols
WB protocol for TRMT1 antibody 14970-1-APDownload protocol
IHC protocol for TRMT1 antibody 14970-1-APDownload protocol
IF protocol for TRMT1 antibody 14970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols