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TRMT61B Polyclonal antibody

TRMT61B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26009-1-AP

Print datasheet

Synonyms

TRMT61B

验证数据展示

  • IHC staining of human colon cancer using 26009-1-AP

    IHC staining of human colon cancer using 26009-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRMT61B antibody included
  • WB analysis of HepG2 using 26009-1-AP

    WB analysis of HepG2 using 26009-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26009-1-AP (TRMT61B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 26009-1-AP

    IHC staining of human colon cancer using 26009-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26009-1-AP (TRMT61B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRMT61B antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRMT61B Ready-To-Use IHC Kit. TRMT61B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

26009-1-AP targets TRMT61B in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TRMT61B fusion protein Ag23307 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tRNA methyltransferase 61 homolog B (S. cerevisiae)
Synonyms TRMT61B
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC010365
Gene Symbol TRMT61B
Gene ID (NCBI) 55006
RRIDAB_2880333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRMT61B antibody 26009-1-APDownload protocol
IHC protocol for TRMT61B antibody 26009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
View Article
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
humanWB

Cell Discov

ALKBH3-dependent m1A demethylation of Aurora A mRNA inhibits ciliogenesis.

Authors - Wenjun Kuang
View Article