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TRPA1 Polyclonal antibody

TRPA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25226-1-AP

Print datasheet

Synonyms

Wasabi receptor, p120, ANKTM1

验证数据展示

  • WB analysis using 25226-1-AP

    WB analysis using 25226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25226-1-AP (TRPA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25226-1-AP targets TRPA1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TRPA1 fusion protein Ag18685 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily A, member 1
Synonyms Wasabi receptor, p120, ANKTM1
Calculated Molecular Weight 1119 aa, 128 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC153003
Gene Symbol TRPA1
Gene ID (NCBI) 8989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPA1 (Transient Receptor Potential Ankyrin 1) is a member of the transient receptor potential (TRP) channel family and is a non-selective cation channel. TRPA1 is predominantly expressed in sensory neurons and is involved in a variety of physiological activities, such as stabilizing cellular membrane potentials, maintaining cellular fluid homeostasis, and regulating intercellular signaling. TRPA1 is a multimodal cell membrane receptor that senses different stimuli and upon activation generates action potential signals via osmolality, temperature and inflammatory factors. TRPA1 has been associated with neurogenic inflammation, osteoarthritis, atopic dermatitis, asthma, inflammatory bowel disease, migraine headache, cancer pain, gout and other diseases, and it is a promising target for drug therapy.

实验方案

Product Specific Protocols
WB protocol for TRPA1 antibody 25226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols