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验证数据展示

  • WB analysis using 24727-1-AP

    WB analysis using 24727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 24727-1-AP

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 24727-1-AP

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TRPC1 Polyclonal antibody

TRPC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24727-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse , rat

应用

WB, IHC, ELISA

别名

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24727-1-AP targets TRPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse , rat
文献引用反应性human
免疫原 TRPC1 fusion protein Ag16488 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily C, member 1
别名 HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1
计算分子量 793 aa, 91 kDa
观测分子量120 kDa
GenBank蛋白编号BC113953
基因名称 TRPC1
Gene ID (NCBI) 7220
RRIDAB_3085750
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48995
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

实验方案

Product Specific Protocols
WB protocol for TRPC1 antibody 24727-1-APDownload protocol
IHC protocol for TRPC1 antibody 24727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Upregulation of TRPC1 protects against high glucose-induced HUVECs dysfunction by inhibiting oxidative stress

Authors - Mengting Qu
View Article