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TRPC4 Polyclonal antibody

TRPC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21349-1-AP

Print datasheet

Synonyms

hTrp 4, HTRP4, Trp related protein 4, TRP4, TRPC4

验证数据展示

  • WB analysis using 21349-1-AP

    WB analysis using 21349-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21349-1-AP (TRPC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21349-1-AP

    IHC staining of human kidney using 21349-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 21349-1-AP

    IHC staining of human kidney using 21349-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21349-1-AP (TRPC4 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21349-1-AP targets TRPC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TRPC4 fusion protein Ag15698 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 4
Synonyms hTrp 4, HTRP4, Trp related protein 4, TRP4, TRPC4
Calculated Molecular Weight 977 aa, 112 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC104725
Gene Symbol TRPC4
Gene ID (NCBI) 7223
RRIDAB_10734426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBN4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRPC4 antibody 21349-1-APDownload protocol
IHC protocol for TRPC4 antibody 21349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Sci Adv

Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.

Authors - Jingjing Duan
View Article
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
View Article